Stainless Steel
Bioreactor System

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BR500 Stainless Steel Bioreactor System

Designed with a compact skid-mounted configuration, BR500 series stainless steel bioreactor excels in ease of installation, mobility, and space utilization. As a sterilization in place (SIP) fermenter, with specified culture vessel from 10 L to 500 L, it's developed for the cultivation of microorganisms and cell cultures.

Key Facts

Configurate specified single vessel: 10 L to 500 L
All-in-one skid-mounted design, easy to install and space-saving
Built-in fixed-speed peristaltic pumps and rotameters
Mature and stable SIP; Precise and automatic pressure control
Application for cell culture (BR500-C1) and microbial fermentation (BR500-M1)

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Features

Compact Skid-mounted Design

Compact skid-mounted configuration enhances ease of installation, mobility, and efficient use of lab space. This design also ensures straightforward setup and relocation while optimizing space utilization, making it ideal for laboratories where space is limited and flexibility is crucial.

Automatic Sterilization-in-Place

Siemens PLC controller automates the SIP process for the culture vessel, gas inlet, and exhaust gas flow paths through advanced program control， which ensures precise and consistent sterilization as well as enhances the safety and reliability of the bioprocess.

Complete Interface Configuration

Tank cover features a comprehensive interface configuration with numerous flexible functional ports for sampling, gas input/output, sensor insertion (pH, DO, temperature), nutrient addition, pressure relief, foam control, and sterile venting, etc.

Classification

BR500-M1 Series

Single Configuration for Microbial Fermentation

BR500-C1 Series

Twin Configuration for Microbial Fermentation

The BR500 series stainless steel bioreactor is designed with a compact, skid-mounted configuration that enhances ease of installation, mobility, and space efficiency. It is available with culture vessels ranging from 10L to 500L, making it versatile for both microbial fermentation and cell culture applications. The unit features built-in fixed-speed peristaltic pumps and rotameters, a user-friendly 10.1-inch touch screen, and advanced SIP (sterilization in place) technology with precise, automatic pressure control. This all-in-one design ensures high performance and adaptability, catering to diverse operational needs.

Suitable for space-constrained working environments
2 options: Applied to microbial fermentation or cell culture
User-friendly HMI, concise design language, simplified operation
Complies with the requirements of a validated GMP environment

Model: BR500-M1-10L ... ... BR500-M1-500L

Culture Vessel: 1 * 10 L, 15 L, 20 L, 30 L, 50 L, 100 L, 150 L, 200 L, 300 L, 500 L

Controller Capacity: Single Vessel

Application: Microbial Fermentation

Model: BR500-C1-10L ... ... BR500-C1-500L

Culture Vessel: 1 * 10 L, 15 L, 20 L, 30 L, 50 L, 100 L, 150 L, 200 L, 300 L, 500 L

Controller Capacity: Single Vessel

Application: Cell Culture

For detailed technical parameters, please contact us or download the specifications.

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Microbial Fermentation

Utilizing two flow paths for air and O2 flow, the flow meter intuitively indicates and allows manual adjustment of the sparger flow rate. O2 is pulsed through a solenoid valve and flows only as needed to maintain the Dissolved Oxygen (DO) set point.

No air is provided at this time. A mass flow controller can be integrated to measure and control the total gas flow range by manual adjustment or automatically combined with a DO controller.

Cell Culture

Simultaneous gassing strategy of overlay and sparger: air goes into overlay through a flow meter and a solenoid valve. Solenoid valves select air, O2, N2 and CO2 to flow to the sparger.

An additional airflow path can be added to the distributor. Mass flowmeter is optional for each gas inlet.

Gassing Strategy Diagram for Microbial Fermentation

Gassing Strategy Diagram for Cell Culture

Complete Software Functions

Siemens PLC controller, stable, reliable and easy to maintain;
Automatic sterilization-in-place (SIP) of the culture vessel, gas inlet and exhaust gas flow path;
Control capacities: Manual/automatic control, PID control, correlation control, step control, custom recipe setting;
Measurement and control opportunities of pH, DO, temperature, foam, feed, gas mixing, agitation, harvest and constant total gas flow control, etc.;
PH control uses time judgment to avoid the mutual addition of CO2 and alkali;
Different software versions, corresponding to C and M series, corresponding to cell culture and fermentation applications;
Real-time online monitoring, data recording, curve display of various parameters;
Automatic storage of operation records, traceability of experimental process,etc.;
3-level password protection ensures data security;
USB (software updates, serial communication), Ethernet (IP Network)

Powerful Hardware Configuration

10.1-inch color touch screen for real-time monitoring and control of bioprocess parameters;
A full set of Hamiltion / Metteler sensors with high precision, supporting repeated sterilization, and facilitating process development and optimization;
Servo Motors: High-precision, maintenance-free, low-noise servo motors for long-term stable operation;
Fast load pump heads for fast and secure handling of tubings;
Individual flow meters with optional TMFC (mass flow controller) precisely adjust gas flow rate;
Quick-connect couplings make it easy to attach all cables and supplies to the culture vessels;

Mature Culture Vessel Design

Material: tank SUS316L (polishing better than Ra 0.4 um), jacket SUS304, insulation SUS304 (aluminum silicate filled)
Stainless steel tank with insulation layer, safe and energy-saving;
Complete tank cover interface configuration, rich and flexible functional ports to meet different process requirements;
Prepared with CIP (Cleaning in Place) interface, allowing the operator to effectively, reproducibly and automatically clean the complete system including the culture vessel, gas inlets, exhaust lines, addition lines and transfer groups;
4-Valve addition array, disposable sampling valves and drain valves are either manually or automatically sterilized;
Equipped with an easy-to detach condenser around the air outlet, for easy cleaning, no effusion and no contaminating bacteria;

Analysis or Control: OUR, CER, KLa, RQ, ORP, methanol, exhaust gas (O2, CO2), glucose, ect.
Gas Supply Control: TMFC control (flow rate - automatic)
Peristaltic Pump: More peristaltic pumps for different functions; upgrade to Watson-Marlow peristaltic pump heads
Scale: Tank weight / replenishing
Light: Light source can be selected from red, blue, and white. Its intensity is adjustable [0-100%]
Drive: Top magnetic drive
Electric lid lifting device enables the easy removal of the lid and allows simple and safe handling during cleaning work or when culture vessel accessories are changed.
Remote Function: Option to upgrade remote monitoring and screen mirroring; Realize remote software upgrade
Different sizes and styles of tanks (mechanical or magnetic stirring, different stirring paddles) to adapt to different process requirements
Dual temperature PID control of jacket and tank ensures stable temperature control and high precision;